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Kex2 Protease from Saccharomyces cerevisiae, Recombinant

Cat No.
NATE-1891
Description
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae. But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
Source
Pichia pastoris
Species
Saccharomyces cerevisiae
Form
White lyophilized
Enzyme Commission Number
EC 3.4.21.61
Activity
>10 unit/mg protein
Molecular Weight
67±6.7 kD
pH Stability
5.0-6.0
Unit Definition
One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25°C with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.
Optimum pH
9
Optimum temperature
37 ºC
Storage
Recommended storage temperature: 2°C-8°C.
Transport condition: blue ice to keep the environment cool.
It should be stored in 20mM NaAc-HAc (pH 5.0-5.5) and 2mM Ca2+. It is stable after 5 cycles freezing and thawing.
Buffer
Recommend storage buffer:pH5.0-5.5 20mM NaAc-HAc buffer and 2mM Ca2+.
Recommend reaction buffer: pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+ .
To prepare 1-10mg/ml Kex2 solution with 20mM NaAc-HAc (pH 5.2) and 2mM Ca2+ for a stored solution, and diluted with reaction buffer shch as pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+. If used after dissolved immediately, with the reaction buffer pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+ to dissolve directly.
Synonyms
KEX2 protease; KEX2; protease; kexin; EC 3.4.21.61
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