Description
Liberase TL Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a low concentration of Thermolysin, a non-clostridial neutral protease.
Applications
Liberase TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
This product has been used for the isolation of pancreatic islets from mice.
Optimum temperature
35-37 °C optimum reaction temp.
Storage
Store unused stock solution in single-use aliquots at -15 to -25 °C. Avoid repeated freezing and thawing!
Synonyms
Liberase TL Research Grade; Liberase TL; Liberase; low Thermolysin concentration