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Matrix Metalloproteinase-1 from Human, Recombinant

Cat No.
NATE-0858
Description
Matrix metalloproteinases are members of a unique family of proteolytic enzymes that have a zinc ion at their active sites and can degrade collagens, elastin and other components of the extracellular matrix (ECM). These enzymes are present in normal healthy individuals and have been shown to have an important role in processes such as wound healing, pregnancy, and bone resorption. However, overexpression and activation of MMPs have been linked with a range of pathological processes and disease states involved in the breakdown and remodeling of the ECM. Such diseases include tumor invasion and metastasis, rheumatoid arthritis, periodontal disease and vascular processes such as angiogenesis, intimal hyperplasia, atherosclerosis and aneurysms. Recently, MMPs have been linked to neurodegenerative diseases such as Alzheimer's, and amyotrophic lateral sclerosis (ALS). Natural inhibitors of MMPs, tissue inhibitor of matrix metalloproteinases (TIMPs) exist and synthetic inhibitors have been developed which offer hope of new treatment options for these diseases.
Abbr
MMP-1, Recombinant (Human)
Alias
MMP-1
Source
Human Rheumatoid Synovial Fibroblast
Species
Human
Product Overview
Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires APMA (30-60 minutes at 37°C, just prior to use) to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar Type I collagen. Must be activated just prior to use.
Form
Liquid
Enzyme Commission Number
EC 3.4.24.7
Activity
>15 mU/mg protein
CAS No.
2593923
Contaminants
no other MMP activity detectable
Molecular Weight
56 kDa/52 kDa
Purity
>90% by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
Storage
< -70°C; Avoid freeze/thaw
Buffer
In 300 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
Synonyms
Matrix Metalloproteinase 1; Human Interstitial Collagenase; Collagenase-1; EC 3.4.24.7; vertebrate collagenase
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CAS No.
Source
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CatalogEXWM-4353
EC No.EC 3.4.24.7
CAS No.9001-12-1
Source
Realted Protocols
LYSOSTAPHIN -Enzymatic Assay Protocol
lysostaphin -Enzymatic Assay Protocol
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