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Native Amyloglucosidase from Aspergillus niger (Glycerol Free)

Cat No.
NATE-1051
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis0 of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glucosidic bonds when the next bond in the sequence is 1,4.
Abbr
Amyloglucosidase, Native (Aspergillus niger) (Glycerol Free)
Source
Aspergillus niger
Applications
This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Total Starch test method.
Form
Buffered solution
Enzyme Commission Number
EC 3.2.1.3
Activity
~ 6520 U/mL (40°C, pH 4.5 on soluble starch)
CAS No.
9032-08-0
Molecular Weight
~ 143,500
Isoelectric point
~ 4.0
pH Stability
4.0-5.5
Specificity
Hydrolysis of terminal (1,4)-linked a-D-glucose residues successively from non-reducing ends of the chains with release of b-D-glucose.
Unit Definition
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 and 40°C
Optimum pH
4
Optimum temperature
70°C
Thermal stability
< 60°C
Stability
> 4 years at 4°C.
Storage
4°C
Preparation Instructions
The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard . The preparation is effectively devoid of cellulase and is free of catalase.
Synonyms
glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase; EC 3.2.1.3; 9032-08-0; glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-190
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-0075
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-0076
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-1079
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceHormoconis resinae
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Realted Service
Realted Protocols
ß-N-ACETYLGLUCOSAMINIDASE -Enzymatic Assay Protocol
HYALURONIDASE -Enzymatic Assay Protocol
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