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Native Aspergillus niger Catalase

Cat No.
NATE-1641
Description
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.
Abbr
CAT, Native (Aspergillus niger)
Source
Aspergillus niger
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.11.1.16
Activity
4,960 U/mg protein, 18,000 U/mL
CAS No.
9001-05-2
Isoelectric point
6.8
pH Stability
6.5-7.5
Specificity
Decomposition of hydrogen peroxide into water and oxygen.
Unit Definition
One unit of catalase activity will decompose 1 micromole of H2O2 per minute at pH 7.0 and 25°C, while the H2O2 concentration falls from 10.3 mM to 9.2 mM. The rate of disappearance of H2O2 is followed by observing the rate of decrease in the absorbance at
240 nm (A240).
Optimum pH
7
Optimum temperature
35°C
Storage
at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in potasssium phosphate buffer (150 mM), pH 7.0. Swirl to mix the enzyme immediately prior to use.
Synonyms
equilase; caperase; optidase; catalase-peroxidase; CAT; H2O2:H2O2 oxidoreductase
Formulation
0.5 mg/ml in 25 mM Sodium Acetate, 50 mM NaCl, pH 5.5 containing 50% glycerol
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