Products

Enzymes for Research, Diagnostic and Industrial Use

Native Aspergillus oryzae Ribonuclease T1

Cat No.
NATE-0658
Description
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3'-GMP and oligonucleotides with a 3'-GMP terminal group.
Abbr
RNase T1, Native (Aspergillus oryzae)
Alias
RNase T1
Source
Aspergillus oryzae
Applications
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies.
Form
ammonium sulfate suspension; Suspension in 2.8 M (NH4)2SO4 solution
Enzyme Commission Number
EC 3.1.27.3
Activity
300,000-600,000 units/mg protein
CAS No.
9026-12-4
Unit Definition
One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.
Storage
−20°C
Warnings
Protein determined by E1%/280
Synonyms
Ribonuclease T1; EC 3.1.27.3; guanyloribonuclease; Aspergillus oryzae ribonuclease; RNase N1; RNase N2; ribonuclease N3; ribonuclease U1; ribonuclease F1; ribonuclease Ch; ribonuclease PP1; ribonuclease SA; RNase F1; ribonuclease C2; binase; RNase Sa; guanyl-specific RNase; RNase G; RNase T1; ribonuclease guaninenucleotido-2'-transferase (cyclizing); ribonuclease N3; ribonuclease N1; 9026-12-4
Download Datasheet:


“RNase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0655
EC No.EC 3.1.27.5
CAS No.9001-99-4
SourceBovine pancreas
CatalogNATE-0656
EC No.EC 3.1.27.5
CAS No.9001-99-4
SourceBovine pancreas
CatalogNATE-0657
EC No.EC 3.1.4.34
CAS No.9050-76-4
SourceE. coli
CatalogNATE-1140
EC No.
CAS No.
SourceHEK293 cells
+ See More >>
Realted Service
Realted Protocols
ribonuclease_u1 -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: