Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
Uricase (Bacillus sp.)
Applications
This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Appearance
White amorphous powder, lyophilized
Activity
GradeⅡ 1.5U/mg-solid or more
Contaminants
Catalase <1.0%
Molecular Weight
approx.150,000
pH Stability
pH 6.0-9.5 (25°C, 20hr)
Michaelis Constant
1.36×10⁻⁵M (Uric acid)
Structure
4 subunits per molecule
Unit Definition
One unit causes the oxidation of one micromole of uric acid per minute under the conditions described below.
Thermal stability
below 60°C (pH 8.0, 10min)
Stability
Stable at -20°C for at least 6 months
Stabilizers
Borate, EDTA, nonionic detergents
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
