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Native Bacillus stearothermophilus Alanine Dehydrogenase

Cat No.
NATE-1899
Description
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation.
Abbr
AlaDH, Native (Bacillus stearothermophilus)
Alias
AlaDH
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for determination of L-alanine.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.4.1.1
CAS No.
9029-06-5
Contaminants
(as AlaDH activity = 100 %)
NADH oxidase: <0.01 %;
Lactate dehydrogenase: <0.10 %.
Molecular Weight
ca. 230,000; Subunit molecular weight : ca. 38,000.
pH Stability
7.0 - 11.5
Michaelis Constant
(125 mM Glycine-NaOH buffer, pH 10.5, at 30 °C)
L-Alanine: 10.0 mM;
NAD+: 0.26 mM.
Specificity
L-Alanine: 100 %;
L-Leucine: 0 %;
L-Isoleucine: 0 %.
Unit Definition
One unit of activity is defined as the amount of AlaDH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
10.4
Thermal stability
No detectable decrease in activity up to 70 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
L-Alanine Dehydrogenase; Alanine dehydrogenase; EC 1.4.1.1; 9029-06-5; AlaDH; NAD+-linked alanine dehydrogenase; alpha-alanine dehydrogenase; NAD+-dependent alanine dehydrogenase; alanine oxidoreductase; NADH-dependent alanine dehydrogenase
Specific Activity
more than 55 U/mg protein
Reaction
L-Alanine + NAD+ + H2O ←→ Pyruvate + NH4+ + NADH
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1801
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceE. coli
CatalogNATE-0043
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceBacillus subtilis
CatalogNATE-0044
EC No.EC 1.4.1.1
CAS No.9029-06-5
SourceE. coli
CatalogEXWM-1442
EC No.EC 1.4.1.1
CAS No.9029-06-5
Source
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Realted Protocols
L-alanine_dehydrogenase -Enzymatic Assay Protocol
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