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Native Bacillus stearothermophilus Phosphoglucose Isomerase

Cat No.
NATE-0553
Description
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes.
Abbr
PGI, Native (Bacillus stearothermophilus)
Alias
PGI
Source
Bacillus stearothermophilus
Applications
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus. The enzyme from Creative Enzymes has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.
Product Overview
The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.
Form
lyophilized powder containing Tris buffer
Enzyme Commission Number
EC 5.3.1.9
Activity
300-1,000 units/mg protein
CAS No.
9001-41-6
Isoelectric point
4.2
pH Stability
42623
Unit Definition
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30°C.
Optimum pH
42623
Storage
−20°C
Synonyms
Glucose-6-phosphate isomerase; EC 5.3.1.9; phosphohexose isomerase; phosphohexomutase; oxoisomerase; hexosephosphate isomerase; phosphosaccharomutase; phosphoglucoisomerase; phosphohexoisomerase; phosphoglucose isomerase; glucose phosphate isomerase; hexose phosphate isomerase; D-glucose-6-phosphate ketol-isomerase; 9001-41-6; PGI
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Related Protocols
phosphoglucose isomerase -Enzymatic Assay Protocol
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