Description
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes.
Abbr
PGI, Native (Baker's Yeast (S. cerevisiae))
Source
Baker's yeast (S. cerevisiae)
Applications
Isomerization of ketoses to aldoses
Enzyme Commission Number
EC 5.3.1.9
Activity
350 U/mg at +25°C with F6P as substrate.
Contaminants
< 0.01% F6P-K, GR, 6-PGDH and PGIuM each < 0.2% B-fructosidase.
Unit Definition
One unit (U) phosphoglucose isomerase will produce 1 mol of glucose-6-phosphate from fructose-6-phosphate in 1 min at +25°C and pH 7.6 (triethanolamine buffer). The above assay produces 1umol of NAD (P)H per umol of glucose-6-phosphate formed.
Storage
Stable at +2 to +8°C.
Buffer
Suspension in 3.2 M ammonium sulfate solution, pH approx. 6
Inhibitors
Pyridoxal-5’-phosphate
Synonyms
Glucose-6-phosphate isomerase; EC 5.3.1.9; phosphohexose isomerase; phosphohexomutase; oxoisomerase; hexosephosphate isomerase; phosphosaccharomutase; phosphoglucoisomerase; phosphohexoisomerase; phosphoglucose isomerase; glucose phosphate isomerase; hexose phosphate isomerase; D-glucose-6-phosphate ketol-isomerase; 9001-41-6; PGI
