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Native Bovine Deoxyribonuclease I


Cat No.
NATE-1875
Description
Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.
Source
Bovine Spleen
Species
Bovine
Form
Freeze-dried powder
Enzyme Commission Number
EC 3.1.21.1
Activity
200 U/mg protein
CAS No.
9003-98-9
Purity
90% (biuret)
Unit Definition
The amount of enzyme which results in an increase in absorbance at 260 nm and 0.001 per minute per ml at 25°C at pH 5.00.
Storage
Store at -20° C
Synonyms
DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; deoxyribonuclease (pancreatic); DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; deoxyribonuclease A; DNA endonuclease; DNA nuclease
Solubility
Distilled water or dilute buffer
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