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Native Bovine Deoxyribonuclease I

Cat No.
PHAM-266
Description
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.2 The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.3 A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with molar ratios being 4:1:1 respectively. Only minor amounts of D are found.4 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)5 and actin6 are known to inhibit the enzyme activity.
Abbr
DNase I, Native (Bovine)
Alias
DNase I
Source
Bovine pancreas
Species
Bovine
Applications
Used for the removal of DNA from protein samples.
Package
vial of >0.5 mg total protein
Enzyme Commission Number
EC 3.1.21.1
CAS No.
9003-98-9
Molecular Weight
mol wt ~31 kDa
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM
Storage
−20°C
Synonyms
EC 3.1.21.1; DNase I; Deoxyribonuclease I; Deoxyribonucleate 5'-oligonucleotido-hydrolase
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