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Native Bovine β-Galactosidase


Cat No.
NATE-0295
Description
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
Abbr
GLB, Native (Bovine)
Alias
β-gal; GLB
Source
Bovine testes
Species
Bovine
Applications
β-Galactosidase from bovine testes was used for coupling to Sepharose.
Form
ammonium sulfate suspension. Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0
Enzyme Commission Number
EC 3.2.1.23
Activity
1.0-3.0 units/mg protein (modified Warburg-Christian)
CAS No.
9031-11-2
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25°C.
Pathway
Defective ALG11 causes ALG11-CDG (CDG-1p), organism-specific biosystem; Defective ALG6 causes ALG6-CDG (CDG-1c), organism-specific biosystem; Defective B4GALT7 causes EDS, progeroid type, organism-specific biosystem
Function
beta-galactosidase activity
Synonyms
β-Galactosidase; beta-gal; β-gal; GLB; 9031-11-2; EC 3.2.1.23; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; S 2107; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat
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Related Protocols
ß-GALACTOSIDASE -Enzymatic Assay Protocol
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