Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Form
A solution in 50% glycerol containing 5mM Tris, 5mM MgCl2 and 0.1mMZnCl2.
Enzyme Commission Number
EC 3.1.3.1
Activity
> 3,000 units per mg protein (37°C, pH9.8, DEA)
Purity
Chromatographically purified, EIA grade.
Unit Definition
One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 37°C, pH 9.8.
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
