Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Applications
This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 1.7.3.3
Activity
GradeⅡ 4.0U/mg-solid or more (containing approx.20% of stabilizers)
Contaminants
Catalase < 1.0%
Molecular Weight
approx. 120 kDa
pH Stability
pH 7.0-11.0 (25°C, 20hr)
Michaelis Constant
2.5×10⁻⁵M (Uric acid)
Structure
4 subunits per molecule (Reactive SH groups are present in the enzyme molecule)
Thermal stability
below 50°C (pH 8.5, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
Borate, EDTA, nonionic detergents
Inhibitors
Heavy metal ions, cyanide,various urate analogs
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
