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Native Cellulomonas sp. Glycerol Dehydrogenase

Cat No.
NATE-0283
Description
Glycerol dehydrogenase is an enzyme in the oxidoreductase family that utilizes the NAD+ to catalyze the oxidation of glycerol to form glycerone (dihydroxyacetone).
Abbr
GDH, Native (Cellulomonas sp.)
Alias
GDH; GlDH; GlyDH
Source
Cellulomonas sp.
Applications
This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.
Form
Lyophilized powder containing bovine serum albumin
Enzyme Commission Number
EC 1.1.1.6
Activity
50-125 units/mg protein
CAS No.
9028-14-2
Molecular Weight
mol wt ~390 kDa
Isoelectric point
4.4 ± 0.1
pH Stability
pH 7.5 – 10.5 (25°C, 20hr)
Michaelis Constant
1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
Structure
10 subunits (42 kDa) per mol of enzyme
Unit Definition
One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25°C at pH 10.0.
Optimum pH
10.0 – 10.5
Optimum temperature
50°C
Thermal stability
below 55°C (pH 7.5, 15min)
Storage
−20°C
Inhibitors
p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co++, Ni++, Cu++, Zn++, Cd++)
Synonyms
EC 1.1.1.6; NAD+-linked glycerol dehydrogenase; glycerol:NAD+ 2-oxidoreductase; GDH; GlDH; GlyDH; 9028-14-2; glycerin dehydrogenase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0284
EC No.EC 1.1.1.6
CAS No.9028-14-2
SourceEnterobacter aerogenes
CatalogEXWM-0345
EC No.EC 1.1.1.6
CAS No.9028-14-2
Source
Realted Service
Realted Protocols
glycerol dehydrogenase -Enzymatic Assay Protocol
ANNITOL DEHYDROGENASE -Enzymatic Assay Protocol
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