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Native Clostridium histolyticum Clostripain

Cat No.
NATE-0143
Description
Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substRate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds. The isoelectric point of the enzyme is 4.8-4.9 (at 8°C), and optimum pH is 7.4~7.8 (against α-benzoyl-arginine ethyl ester).
Abbr
Clostripain, Native (Clostridium histolyticum)
Alias
Clostripain
Source
Clostridium histolyticum
Form
lyophilized powder
Enzyme Commission Number
EC 3.4.22.8
Activity
> 20 units/mg solid
CAS No.
9028-00-6
Unit Definition
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of 2.5 mM DTT.
Storage
2-8°C
Synonyms
clostridiopeptidase B; clostridium histolyticum proteinase B; α-clostridipain; clostridiopeptidase; Endoproteinase Arg-C; EC 3.4.22.8; 9028-00-6
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