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Native Escherichia coli Acetate Kinase

Cat No.
NATE-0017
Description
In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. Short-chain fatty acids (SCFAs) play a major role in carbon cycle and can be utilized as a source of carbon and energy by bacteria. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydRate. A related enzyme, butyRate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyRate in the presence of ATP to form butyryl phosphate
Abbr
Acetate kinase, Native (E. coli)
Alias
Acetate kinase
Source
Escherichia coli
Applications
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylatio.
Form
Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol
Enzyme Commission Number
EC 2.7.2.1
Activity
> 150 units/mg protein (biuret)
CAS No.
9027-42-3
Unit Definition
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25°C.
Storage
−20°C
Pathway
Carbon metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Propanoate metabolism, organism-specific biosystem
Synonyms
Acetate kinase (phosphorylating); Acetic kinase; Acetokinase; AK; EC 2.7.2.1; 9027-42-3; Acetate kinase

"Acetate kinase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1826 Acetate Kinase (Crude Enzyme) EC 2.7.2.1 9027-42-3 E. coli Inquiry
NATE-0016 Native Bacillus stearothermophilus Acetate Kinase EC 2.7.2.1 9027-42-3 Bacillus stearothermophilus Inquiry
EXWM-3167 acetate kinase EC 2.7.2.1 9027-42-3 Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

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