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Native Escherichia coli Penicillin Amidase

Cat No.
NATE-0541
Description
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression. It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.
Abbr
Penicillin Amidase, Native (E. coli)
Source
Escherichia coli
Applications
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N-acylhomoserine lactones) in degradation of antibiotics. It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp. Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.
Product Overview
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.
Form
Type II, ammonium sulfate suspension, Suspension in 0.1 M phosphate, pH 7.5 and 3 M ammonium sulfate.
Enzyme Commission Number
EC 3.5.1.11
Activity
Type I, 5-10 units/mg protein; Type II, > 10 units/mg protein (E1%/280).
CAS No.
9014-06-6
Molecular Weight
Mr ~70 kDa
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Storage
2-8°C
Warnings
Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin
Synonyms
penicillin amidase; penicillin acylase; benzylpenicillin acylase; novozym 217; semacylase; α-acylamino-β-lactam acylhydrolase; ampicillin acylase; EC 3.5.1.11; 9014-06-6
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogEXWM-4389
EC No.EC 3.5.1.11
CAS No.9014-06-6
Source
Realted Protocols
penicillin amidase -Enzymatic Assay Protocol
penicillin amidase -Enzymatic Assay Protocol
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