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Native Glutamine Synthetase from Microorganism

Cat No.
DIA-411
Description
Glutamine synthetase (GS) (EC 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine: Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by nitrate reduction, amino acid degradation, and photorespiration. The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.
Abbr
GS, Native (Microorganism)
Alias
GS
Source
Microorganism
Appearance
Light yellow lyophilizate
Enzyme Commission Number
EC 6.3.1.2
Activity
> 7 U/mg lyophilizate
CAS No.
9023-70-5
Contaminants
catalase < 0.5%
Molecular Weight
ca. 900 kDa
Isoelectric point
6.5
pH Stability
6.5–9.5
Michaelis Constant
1.5 x 10^-2 M (L-glutamate) 1.3 x 10^-4 M (ammonia) 8.7 x 10^-4 M (ATP)
Structure
57 kDa (SDS-PAGE)
Specificity
L-glutamate (100), D-glutamate (0.8), NH3 (100), NH2OH (12), ATP (100), GTP (2.5)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of phosphate per min at 37°C and pH 7.0.
Optimum pH
7
Optimum temperature
60°C
Activators
Mg2+, Mn2+
Thermal stability
below 40°C
Storage
at -20°C
Stabilizers
Sucrose
Inhibitors
methionine sulfoximine, carbamyl phosphate
Synonyms
Glutamine synthetase; GS; EC 6.3.1.2; Glutamate-ammonia ligase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1675
EC No.EC 6.3.1.2
CAS No.9023-70-5
SourceE. coli
CatalogDIA-155
EC No.EC 6.3.1.2
CAS No.9023-70-5
SourceBacillus sp.
CatalogNATE-0321
EC No.EC 6.3.1.2
CAS No.9023-70-5
SourceEscherichia coli
CatalogEXWM-5722
EC No.EC 6.3.1.2
CAS No.9023-70-5
Source
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Realted Protocols
GLUTAMINE SYNTHETASE -Enzymatic Assay Protocol
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