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Native Glycine max (soybean) Lipoxidase


Cat No.
NATE-0407
Description
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Abbr
Lipoxygenase, Native (Glycine max (soybean))
Source
Glycine max (soybean)
Applications
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12-or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Form
Type I, ammonium sulfate suspension, Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0; Type II, lyophilized powder, Contains stabilizer and NaCl.
Enzyme Commission Number
EC 1.13.11.12
Activity
Type I, 500,000-1,000,000 units/mg protein; Type II, > 50,000 units/mg solid.
CAS No.
9029-60-1
Molecular Weight
mol wt 108 kDa (two 54 kDa subunits)
Unit Definition
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25°C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Storage
2-8°C
Warnings
Prepared by affinity chromatography. Protein determined by biuret.
Synonyms
Lipoxygenases; EC 1.13.11.12; 9029-60-1; 13-lipoxidase; carotene oxidase; 13-lipoperoxidase; fat oxidase; 13-lipoxydase; lionoleate:O2 13-oxidoreductase; linoleate 13S-lipoxygenase
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Related Services
Related Protocols
lipoxidase -Enzymatic Assay Protocol
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