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Enzymes for Research, Diagnostic and Industrial Use

Native Guar α-Galactosidase

Cat No.
NATE-1073
Description
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).
Abbr
GLA, Native (Guar)
Alias
GLA
Source
Guar seed
Species
Guar
Applications
High purity alpha-Galactosidase (Guar) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.22
Activity
> 50 U/mg
CAS No.
9025-35-8
Molecular Weight
44500
Concentration
500 U/ml
Isoelectric point
3.7-4.1
pH Stability
4.0-8.0
Unit Definition
One Unit of activity is the amount of enzyme required to release one micromole of product (e.g. p-nitrophenyl) per min at pH 4.5 and 40°C.
Optimum pH
4.5-5.0
Optimum temperature
40°C
Thermal stability
Unstable above 40°C
Stability
> 4 years at 4°C.
Storage
Stored at 4°C. BSA (0.5 mg/mL) is added to improve stability properties. On dissolution in buffer, the enzyme should be stored in the frozen state in a polypropylene container between use. We recommend the addition of BSA (0.5 mg/mL) to all dilution buffers.
Synonyms
Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase
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