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Native Porcine Citrate Synthase


Cat No.
NATE-0166
Description
Citrate synthase catalyses the conversion of Citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Abbr
CS, Native (Porcine)
Alias
CS; Citrate Synthase
Source
Porcine heart
Species
Porcine
Form
ammonium sulfate suspension. Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Enzyme Commission Number
EC 4.1.3.7
Activity
> 100 units/mg protein
CAS No.
9027-96-7
Unit Definition
One unit will form 1.0 μmole of Citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37°C.
Storage
2-8°C
Buffer
H2O: soluble 1.0 mg/mL, clear
Pathway
2-Oxocarboxylic acid metabolism, organism-specific biosystem; Biosynthesis of amino acids, conserved biosystem; Citrate cycle (TCA cycle), organism-specific biosystem
Function
Citrate (Si)-synthase activity
Synonyms
CS; EC 4.1.3.7; EC 2.3.3.1; 9027-96-7; Citrate (Si)-synthase; (R)-citric synthase; Citrate oxaloacetate-lyase [(pro-3S)-CH2COO-->acetyl-CoA]
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Catalog
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CatalogNATE-1059
EC No.EC 2.3.3.1
CAS No.9027-96-7
SourceE. coli
CatalogNATE-0165
EC No.EC 4.1.3.7
CAS No.9027-96-7
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Related Protocols
CITRATE SYNTHASE -Enzymatic Assay Protocol
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