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Native Porcine Deoxyribonuclease II

Cat No.
NATE-0202
Description
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. In vitro, its optimum pH range is 4.5-5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6-5.9. The molecular weight is approximately 38 kDa Da.
Abbr
DNASE2, Native (Porcine)
Alias
DNase II alpha; DNASE2
Source
Porcine spleen
Species
Porcine
Applications
DNase II from Creative Enzymes has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
Form
lyophilized powder. Contains sodium chloride
Enzyme Commission Number
EC 3.1.22.1
Activity
2,000-6,000 Kunitz units/mg protein (biuret)
CAS No.
9025-64-3
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM
Storage
−20°C
Pathway
Lysosome, organism-specific biosystem; Lysosome, conserved biosystem
Function
deoxyribonuclease II activity
Synonyms
DNASE2; deoxyribonuclease II; EC 3.1.22.1; 9025-64-3; DNase II; pancreatic DNase II; deoxyribonucleate 3'-nucleotidohydrolase; DNase II; pancreatic DNase II; acid deoxyribonuclease; acid Dnase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0201
EC No.EC 3.1.22.1
CAS No.9025-64-3
SourceBovine spleen
CatalogEXWM-3580
EC No.EC 3.1.22.1
CAS No.9025-64-3
Source
Realted Service
Realted Protocols
DEOXYRIBONUCLEASE II -Enzymatic Assay Protocol
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