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Native Porcine Prolidase

Cat No.
NATE-0627
Description
Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
Abbr
Prolidase, Native (Porcine)
Alias
prolidase
Source
Porcine kidney
Species
Porcine
Applications
Prolidase is has an important role recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.
Form
Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.
Enzyme Commission Number
EC 3.4.13.9
Activity
> 100 units/mg protein
CAS No.
9025-32-5
Composition
Protein, 20-60% Lowry
Unit Definition
One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40°C.
Storage
−20°C
Synonyms
Xaa-Pro dipeptidase; prolidase; imidodipeptidase; proline dipeptidase; peptidase D; gamma-peptidase; X-Pro dipeptidase; EC 3.4.13.9; 9025-32-5
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1782
EC No.EC 3.4.13.9
CAS No.9025-32-5
SourceE.coli
CatalogEXWM-4042
EC No.EC 3.4.13.9
CAS No.9025-32-5
Source
Realted Protocols
prolidase -Enzymatic Assay Protocol
PROLIDASE -Enzymatic Assay Protocol
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