Products

Enzymes for Research, Diagnostic and Industrial Use

Native Proteus sp. Glutamate Dehydrogenase (NADP-dependent)

Cat No.
DIA-196
Description
Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate.
Abbr
GLDH (NADP) (Proteus sp.)
Alias
GLDH
Source
Proteus sp.
Applications
This enzyme is useful for enzymatic determination of NH₃, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.
Appearance
Solution with 50mM Tris-HCl buffer containing 0.05% NaN₃ and 5.0mM EDTA, pH 7.8
Enzyme Commission Number
EC 1.4.1.4
Activity
GradeⅡ∙Ⅲ 300U/mg-protein or more (9,000U/ml or more)
CAS No.
2604121
Contaminants
NADPH oxidase < 1.0×10⁻²% Glutathione reductase < 1.0×10⁻²% (GradeⅡ-209) < 1.0×10⁻¹% (GradeⅢ-309)
Molecular Weight
approx. 300 kDa
Isoelectric point
4.6
pH Stability
pH 6.0-8.5 (25°C, 20hr)
Michaelis Constant
1.1×10⁻³M (NH₃), 3.4×10⁻⁴M (α-Ketoglutarate), 1.2×10⁻³M (L-Glutamate), 1.4×10⁻⁵M (NADPH), 1.5×10⁻⁵M (NADP⁺)
Structure
6 subunits (M.W.50,000) per mol of enzyme
Optimum pH
8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)
Optimum temperature
45°C (α-KG→L-Glu) 45-55°C (L-Glu→α-KG)
Thermal stability
below 50°C (pH 7.4, 10min)
Stability
Stable at 5°C for at least 6 months
Stabilizers
Ethylenediaminetetraacetic acid (EDTA)
Inhibitors
Hg⁺⁺, Cd⁺⁺, p-chloromercuribenzoate, pyridine, 4-4'-dithiopyridine, 2,2'-dithiopyridine
Synonyms
glutamate dehydrogenase (NADP+); glutamic dehydrogenase; dehydrogenase; glutamate (nicotinamide adenine dinucleotide (phosphate)); glutamic acid dehydrogenase; L-glutamate dehydrogenase; L-glutamic acid dehydrogenase; NAD(P)-glutamate dehydrogenase; NAD(P)H-dependent glutamate dehydrogenase; glutamate dehydrogenase (NADP); EC 1.4.1.4; GLDH
Download Datasheet:


“GLDH” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1701
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceE. coli
CatalogNATE-1802
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceE. coli
CatalogDIA-146
EC No.EC 1.4.1.3
CAS No.9001-46-1
SourceBovine liver
CatalogDIA-197
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceMicroorganism
+ See More >>
Realted Protocols
L-glutamic dehydrogenase (β-nadp) -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: