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Enzymes for Research, Diagnostic and Industrial Use

Native Pseudomonas fragi Acyl-CoA Synthetase

Cat No.
NATE-0027
Description
The Long chain fatty acyl-CoA synthetase enzyme is a member of the ligase family that activates the breakdown of complex fatty acids. Long chain fatty acyl-CoA synthetase plays a crucial role in intermediary metabolism by catalyzing the formation of fatty acyl-CoA by a two-step process proceeding through an adenylated intermediate. It is an enzyme present in all organisms from bacteria to humans. It catalyzes the pre-step reaction for β-oxidation of fatty acids or can be incorporated in phospholipids.
Abbr
ACS, Native (Pseudomonas fragi)
Alias
ACS
Source
Pseudomonas fragi
Applications
Useful in the enzymatic determination of fatty acid when coupled with Acyl-CoA oxidase
Appearance
White powder
Product Overview
ACS is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Form
Freeze dried powder
Enzyme Commission Number
EC 6.2.1.3
Activity
2-8 U/mg
CAS No.
9013-18-7
Contaminants
Catalase < 1.00%; Glucose oxidase < 0.1%
Molecular Weight
60 kDa (Sephadex G‒150) SDS‒PAGE 62 kDa
Isoelectric point
pH 5.2
pH Stability
6.0-8.0 (37°C, 2 hrs)
Michaelis Constant
Palmitic acid 1.1 × 10-5M; ATP 1.7 × 10-4M; CoA 3.2 × 10-4M
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of fatty acid to acyl‒CoA per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
7.7
Activators
Triton X‒100
Thermal stability
Stable at 50°C and below (pH 7.5, 10 mins)
Stability
At least one year at-20°C
Storage
Keep in freezer (-20°C to-80°C), dry place in well-closed containers and away from direct sun light).
Stabilizers
ATP
Synonyms
EC 6.2.1.3; ACS; acyl-CoA synthetase; fatty acid thiokinase (long chain); acyl-activating enzyme; palmitoyl-CoA synthase; lignoceroyl-CoA synthase; arachidonyl-CoA synthetase; acyl coenzyme A synthetase; acyl-CoA ligase; palmitoyl coenzyme A synthetase; thiokinase; palmitoyl-CoA ligase; acyl-coenzyme A ligase; fatty acid CoA ligase; long-chain fatty acyl coenzyme A synthetase; oleoyl-CoA synthetase; stearoyl-CoA synthetase; long chain fatty acyl-CoA synthetase; long-chain acyl CoA synthetase; fatty acid elongase; LCFA synthetase; pristanoyl-CoA synthetase; ACS3; long-chain acyl-CoA synthetase I; long-chain acyl-CoA synthetase II; fatty acyl-coenzyme A synthetase; long-chain acyl-coenzyme A synthetase; FAA1
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1682
EC No.EC 6.2.1.3
CAS No.9013-18-7
Source
CatalogNATE-1712
EC No.EC 6.2.1.3
CAS No.9013-18-7
SourceMicroorganism
CatalogNATE-0028
EC No.EC 6.2.1.3
CAS No.9013-18-7
SourcePseudomonas sp.
CatalogEXWM-5687
EC No.EC 6.2.1.3
CAS No.9013-18-7
Source
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Realted Protocols
acyl_coenzyme_a_synthetase -Enzymatic Assay Protocol
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