Description
Isoamylase is an enzyme with system name glycogen 6-alpha-D-glucanohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins. This enzyme also readily hydrolyses amylopectin.
Abbr
Isoamylase, Native (Pseudomonas sp.)
Form
ammonium sulfate suspension; Supplied as a suspension in 3.2 M ammonium sulfate
Enzyme Commission Number
EC 3.2.1.68
Activity
180 U/mg protein (on oyster glycogen) at pH 4.0 and 40°C. 200 U/mL
Purity
Single major band on SDS-gel electrophoresis (MW = 71,500).
Unit Definition
One Unit of isoamylase activity is defined as the amount of enzyme required to release one μmole of reducing sugar per minute from oyster glycogen (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C. One Unit as defined here is approximately equal to 67 KU as defined by Sigma for Isoamylase (rice starch as substrate at pH 3.5 and 40°C).
Synonyms
EC 3.2.1.68; debranching enzyme; glycogen alpha-1,6-glucanohydrolase; isoamylase
