Description
Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
Abbr
Pyruvate Kinase, Native (Thermophillic bacteria)
Source
Thermophillic bacteria
Applications
ATP regeneration in biocatalysis.
Enzyme Commission Number
EC 2.7.1.40
Unit Definition
One unit is defined as the amount of enzyme oxidizing 1 µmol of NADH per one minute from phosphoenolpyruvic acid as a substrate, using ε340=6.22 mM-1cm-1.
Thermal stability
100% stability after 1 hour at 85°C
Buffer
50 mM Tris-HCl (pH 7.5), 50 mM NaCl
Synonyms
Pyruvate kinase; EC 2.7.1.40; 9001-59-6; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; pyruvate kinase (phosphorylating); fluorokinase; fluorokinase (phosphorylating); pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase
