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Native Rhizopus sp. Amyloglucosidase

Cat No.
NATE-0076
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis0 of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glucosidic bonds when the next bond in the sequence is 1,4.
Abbr
Amyloglucosidase, Native (Rhizopus sp.)
Alias
γ-amylase; acid maltase; glucose amylase
Source
Rhizopus sp.
Applications
Amyloglucosidase is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Rhizopus sp., has been used to study the cleavage of oligosaccharides during ER-associated degradation of proteins (ERAD). The enzyme has been used in the glycosylation of N-vanillyl-nonanamide to form a water-soluble component with pharmacological applications. The glycogen was estimated in stipe residue of Coprinus cinereus by treating with amyloglucosidase and measuring the amount of glucose produced.
Form
Lyophilized salt free powder
Enzyme Commission Number
EC 3.2.1.3
Activity
> 40,000 units/g solid
CAS No.
9032-08-0
Unit Definition
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55°C.
Storage
−20°C
Buffer
0.03 M sodium Citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL
Synonyms
glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase; EC 3.2.1.3; 9032-08-0
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-190
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-0075
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-1051
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-1079
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceHormoconis resinae
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Realted Service
Realted Protocols
ß-N-ACETYLGLUCOSAMINIDASE -Enzymatic Assay Protocol
HYALURONIDASE -Enzymatic Assay Protocol
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