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Native Sphingomyelinase from Streptomyces sp.

Cat No.
NATE-1160
Description
Sphingomyelin phosphodiesterase is a hydrolase enzyme that is involved in sphingolipid metabolism reactions. SMase is a member of the DNase I superfamily of enzymes and is responsible for breaking sphingomyelin (SM) down into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stresses.
Abbr
SMase, Native (Streptomyces sp.)
Alias
SMase
Source
Streptomyces sp.
Applications
This enzyme is useful for enzymatic determination of sphingomyelin when coupled With alkaline phosphatase and choline oxidase.
Appearance
White to brownish amorphous powder,lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 3.1.4.12
Activity
> 500 U/mg
CAS No.
9031-54-3
Molecular Weight
37.5 kDa (SDS‒PAGE)
Isoelectric point
8.6
pH Stability
5.0‒8.0
Michaelis Constant
Sphingomyelin 0.45 × 10‒3M
Unit Definition
One unit is defined as the amount of enzyme which hydrolyzes 1 μmole of sphingomyelin per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
7.0‒8.0
Activators
Mg2+, Mn2+, Non‒ionic detergents
Thermal stability
Stable at 40°C and below
Stability
At least one year at -20°C
Storage
Storage at -20°C in the presence of a desiccant is recommended.
Stabilizers
Mg2+
Inhibitors
EDTA
Synonyms
Sphingomyelin phosphodiesterase; EC 3.1.4.12; neutral sphingomyelinase; 9031-54-3; sphingomyelin cholinephosphohydrolase; sphingomyelinase; Smase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0672
EC No.EC 3.1.4.12
CAS No.9031-54-3
SourceBacillus cereus
CatalogNATE-0673
EC No.EC 3.1.4.12
CAS No.9031-54-3
SourceStaphylococcus aureus
CatalogEXWM-3712
EC No.EC 3.1.4.12
CAS No.9031-54-3
Source
Realted Service
Realted Protocols
SPHINGOMYELINASE -Enzymatic Assay Protocol
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