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Native Spinach Aldolase

Cat No.
NATE-0049
Description
Fructose-bisphosphate aldolase (EC 4.1.2.13), often just aldolase, is an enzyme catalyzing a reversible reaction that splits the aldol, fructose 1,6-bisphosphate, into the triose phosphates dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P). Aldolase can also produce DHAP from other (3S,4R)-ketose 1-phosphates such as fructose 1-phosphate and sedoheptulose 1,7-bisphosphate. Gluconeogenesis and the Calvin cycle, which are anabolic pathways, use the reverse reaction. Glycolysis, a catabolic pathway, uses the forward reaction. Aldolase is divided into two classes by mechanism.
Abbr
Aldolase, Native (Spinach)
Alias
aldolase
Source
Spinach
Product Overview
Protein determined by Lowry
Form
lyophilized powder.
Enzyme Commission Number
EC 4.1.2.13
Activity
0.3-1.0 units/mg protein
CAS No.
9024-52-6
Unit Definition
One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25°C.
Storage
−20°C
Synonyms
aldolase; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase; fructose diphosphate aldolase; D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase; EC 4.1.2.13; 9024-52-6
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Related Protocols
aldolase -Enzymatic Assay Protocol
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