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Non-specific endo-1,3(4) β-Glucanase from Clostridium thermocellum, Recombinant

Cat No.
NATE-1076
Description
β-glucanases degrade β-1,4-glucans of cellulose, xyloglucan and β-1,4-xylan. β-Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan. It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.
Abbr
β-Glucanase, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum
Applications
High purity recombinant Non-specific endo-1,3(4) beta-Glucanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.6
Activity
314 U/mg protein (on barley β-glucan) at pH 6.5 and 40°C; 494 U/mg protein (on barley β-glucan) at pH 6.5 and 60°C.
CAS No.
62213-14-3
Molecular Weight
~ 29,300
Isoelectric point
~ 4.9
pH Stability
4.0 - 9.0
Unit Definition
One Unit of glucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from barley β-glucan (5 mg/mL) in sodium phosphate buffer (100 mM) pH 6.5.
Optimum pH
6.5
Optimum temperature
60°C
Thermal stability
up to 50°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.5 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
endo-1,3-β-D-glucanase; laminarinase; laminaranase; β-1,3-glucanase; β-1,3-1,4-glucanase; endo-1,3-β-glucanase; endo-β-1,3 (4)-glucanase; endo-β-1,3-1,4-glucanase; endo-β-(1→3)-D-glucanase; endo-1,3-1,4-β-D-glucanase; endo-β-(1-3)-D-glucanase; endo-β-1,3-glucanase IV; endo-1,3-β-D-glucanase; 1,3-(1,3; 1,4)-β-D-glucan 3 (4)-glucanohydrolase; EC 3.2.1.6
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Realted Protocols
mycodextranase -Enzymatic Assay Protocol
LAMINARINASE -Enzymatic Assay Protocol
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