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O-Glycosidase from Enterococcus faecalis, Recombinant

Cat No.
NATE-0905
Description
O-Glycosidase releases unsubstituted Ser-and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase.
Abbr
O-Glycosidase, Recombinant (E. faecalis)
Source
Enterococcus faecalis
Applications
High purity recombinant endo-alpha-N-Acetylgalactosaminidase (E. faecalis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.97
Activity
3.0 U/mg protein (on Gal-β-1,3-GalNAc-α-pNP) at pH 7.5 and 37°C
CAS No.
59793-96-3
Molecular Weight
~ 158,800 Da
Isoelectric point
~ 5.2
Specificity
Hydrolysis of the O-glycosidic α-linkage between Gal-β1,3-GalNAc (Core 1) or GlcNAc-β1,3GalNAc (Core 3) and a serine or threonine residue of glycopeptides and glycoproteins.
Unit Definition
One Unit of endo-α-N-acetylgalactosaminidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from Gal-β1,3-GalNAc-α-pNP (0.2 mM) in Tris.HCl buffer (10 mM) pH 7.5 and 37°C, monitored at 410 nm.
Optimum pH
7.5
Storage
Store at -20°C
Buffer
20 mM Tris.HCl pH 7.5, 50 mM NaCl, 5 mM EDTA plus 0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in Tris.HCl buffer (100 mM), pH 7.5 containing 1 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
endo-α-acetylgalactosaminidase; endo-α-N-acetyl-D-galactosaminidase; mucinaminylserine mucinaminidase; D-galactosyl-3-(N-acetyl-α-D-galactosaminyl)-L-serine mucinaminohydrolase; endo-α-GalNAc-ase; glycopeptide α-N-acetylgalactosaminidase; D-galactosyl-N-acetyl-α-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase; EC 3.2.1.97
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ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0497
EC No.EC 3.2.1.97
CAS No.9032-92-2
SourceE. coli
CatalogEXWM-3960
EC No.EC 3.2.1.97
CAS No.59793-96-3
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