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Oligo-α-(1,4-1,6)-glucosidase from Bacillus sp., Recombinant

Cat No.
NATE-0906
Description
Sucrase-isomaltase is a glucosidase enzyme located in on the brush border of the small intestine with system name oligosaccharide 6-alpha-glucohydrolase. Sucrase-isomaltase is a type II transmembrane glycoprotein located in the brush border of the small intestine. It has preferential expression in the apical membranes of enterocytes. The enzyme's purpose is to digest dietary carbohydrates such as starch, glucose, and isomaltose. By further processing the broken-down products, energy in the form of ATP can be generated.
Abbr
Oligo-1,6-Glucosidase, Recombinant (Bacillus sp.)
Source
Bacillus sp.
Applications
High purity oligo-α-(1,4-1,6)-glucosidase (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.10
Activity
87 U/mg protein (on p-nitrophenyl-α-D-glucopyranoside) at pH 7.0 and 40°C.
Molecular Weight
~ 66.9 kDa
Isoelectric point
~ 5.4
pH Stability
6.0-9.0 (> 75% control activity after 24 hours at 4°C)
Specificity
Hydrolysis of terminal non-reducing α-(1,4-1,6)-linked D-glucose residues in oligosaccharides from some oligosaccharides produced from starch and glycogen.
Unit Definition
One Unit of oligo-α-(1,4-1,6)-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 40°C.
Optimum pH
7
Optimum temperature
40°C
Stability
> 2 years at 4°C.
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 7.0 containing 1 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.10; oligo-1,6-glucosidase; limit dextrinase; isomaltase; exo-oligo-1,6-glucosidase; dextrin 6alpha-glucanohydrolase; alpha-limit dextrinase; dextrin 6-glucanohydrolase; oligosaccharide alpha-1,6-glucohydrolase; Sucrase-isomaltase
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Realted Protocols
keratanase -Enzymatic Assay Protocol
isomaltase -Enzymatic Assay Protocol
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