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Oligo-α-1,6-Glucosidase from Microbial, Recombinant

Cat No.
NATE-0907
Description
Sucrase-isomaltase is a glucosidase enzyme located in on the brush border of the small intestine with system name oligosaccharide 6-alpha-glucohydrolase. Sucrase-isomaltase is a type II transmembrane glycoprotein located in the brush border of the small intestine. It has preferential expression in the apical membranes of enterocytes. The enzyme's purpose is to digest dietary carbohydrates such as starch, glucose, and isomaltose. By further processing the broken-down products, energy in the form of ATP can be generated.
Abbr
Oligo-1,6-Glucosidase, Recombinant (Microbial)
Source
Microbial
Applications
High purity recombinant Oligo-alpha-1,6-Glucosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.10
Activity
321 U/mg protein (on p-nitrophenyl-α-D-glucopyranoside) at pH 4.5 and 50°C; 171 U/mg protein (on p-nitrophenyl-α-D-glucopyranoside) at pH 4.5 and 40°C.
Molecular Weight
~ 67.3 kDa
Isoelectric point
~ 5.4
pH Stability
5.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Unit Definition
One Unit of oligo-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5.
Optimum pH
4.5
Optimum temperature
50°C
Stability
> 4 years at 4°C.
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (50 mM), pH 4.5 containing 1 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.10; oligo-1,6-glucosidase; limit dextrinase; isomaltase; exo-oligo-1,6-glucosidase; dextrin 6alpha-glucanohydrolase; alpha-limit dextrinase; dextrin 6-glucanohydrolase; oligosaccharide alpha-1,6-glucohydrolase; Sucrase-isomaltase
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Realted Protocols
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