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Pectate Lyase from Cellvibrio japonicus, Recombinant

Cat No.
NATE-0909
Description
Pectate lyase (EC 4.2.2.2) is an enzyme involved in the maceration and soft rotting of plant tissue. Pectate lyase is responsible for the eliminative cleavage of pectate, yielding oligosaccharides with 4-deoxy-α-D-mann-4-enuronosyl groups at their non-reducing ends. The protein is maximally expressed late in pollen development. It has been suggested that the pollen expression of pectate lyase genes might relate to a requirement for pectin degradation during pollen tube growth. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
Pectate Lyase, Recombinant (C. japonicus)
Source
Cellvibrio japonicus
Applications
High purity recombinant Pectate lyase (C. japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.2.2
Activity
690 U/mg protein (on polygalacturonic acid) at pH 10.0 and 40°C.
CAS No.
9015-75-2
Molecular Weight
~ 38,035 Da
Isoelectric point
~ 9.3
pH Stability
6.5 - 10.5
Unit Definition
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of 4,5-unsaturated product per minute from polygalacturonic acid (1.25 mg/ml) in the presence of calcium chloride (1 mM) in CAPS buffer (50 mM) pH 10.0 and 40°C, monitored at 235 nm.
Optimum pH
10
Optimum temperature
62°C
Stability
> 4 years at 4°C.
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in CAPS buffer (50 mM), pH 10.0 containing calcium chloride (1 mM). Swirl to mix the enzyme immediately prior to use.
Synonyms
(1->4)-alpha-D-galacturonan lyase; polygalacturonic transeliminase; pectic acid transeliminase; polygalacturonate lyase; endopectin methyltranseliminase; pectate transeliminase; endogalacturonate transeliminase; pectic acid lyase; pectic lyase; alpha-1,4-D-endopolygalacturonic acid lyase; PGA lyase; PPase-N; endo-alpha-1,4-polygalacturonic acid lyase; polygalacturonic acid lyase; pectin trans-eliminase; Polygalacturonic acid trans-eliminase; Pectate lyase; EC 4.2.2.2
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