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Phosphogluconate dehydrogenase from Human, Recombinant

Cat No.
NATE-1652
Description
In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+↔ D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
6PGD, Recombinant (Human)
Source
E. coli
Species
Human
Product Overview
This protein is fused with 6x His tag at N terminus and the protein has a calculated MW of 55.3 kDa (503aa).
Form
Liquid
Enzyme Commission Number
EC 1.1.1.44
Activity
>10 units/mg
Molecular Weight
53.3 kDa
Purity
> 90% by SDS-PAGE
Concentration
1 mg/ml
Unit Definition
One unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25°C, in the presence of ß-NADP.
Storage
Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.
Synonyms
6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating
Formulation
1 mg/ml solution in 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA and 10% glycerol.
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0008
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceSheep liver
CatalogNATE-0009
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceYeast
CatalogNATE-0796
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceE. coli
CatalogNATE-1167
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceE. coli
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Realted Protocols
6-PHOSPHOGLUCONIC DEHYDROGENASE -Enzymatic Assay Protocol
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