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Leaf-branch Compost Poly (ethylene terephthalate) Hydrolase
Cat No.
EXWM-3437
Description
PETases are an esterase class of enzymes that catalyze the hydrolysis of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl terephthalate (MHET). The idealized chemical reaction is (where n is the number of monomers in the polymer chain):
(ethylene terephthalate)n + H2O → (ethylene terephthalate)n-1 + MHET
Trace amount of the PET breaks down to bis(2-hydroxyethyl) terephthalate (BHET). PETases can also break down PEF-plastic (polyethylene-2,5-furandicarboxylate), which is a bioderived PET replacement. PETases can't catalyze the hydrolysis of aliphatic polyesters like polybutylene succinate or polylactic acid.
Non-enzymatic natural degradation of PET will take hundreds of years, but PETases can degrade PET in matter of days.
(ethylene terephthalate)n + H2O → (ethylene terephthalate)n-1 + MHET
Trace amount of the PET breaks down to bis(2-hydroxyethyl) terephthalate (BHET). PETases can also break down PEF-plastic (polyethylene-2,5-furandicarboxylate), which is a bioderived PET replacement. PETases can't catalyze the hydrolysis of aliphatic polyesters like polybutylene succinate or polylactic acid.
Non-enzymatic natural degradation of PET will take hundreds of years, but PETases can degrade PET in matter of days.
Source
E. coli
Form
Freeze-dried powder
Enzyme Commission Number
EC 3.1.1.101
Activity
194U/mg
Molecular Weight
27.6 kDa
Purity
>95% by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme that will generate 1.0 mmol TNB per minute at 50 °C.
Storage
Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Buffer
20mM Tris, pH8.0, 300mM NaCl, 200mM imidazole and 3% trehalose
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