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Polynucleotide phosphorylase from Synechocystis sp., Recombinant

Cat No.
NATE-0610
Description
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Abbr
PNPase, Recombinant (Synechocystis sp.)
Alias
PNPase
Source
E. coli
Species
Synechocystis sp.
Applications
Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.
Product Overview
Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly (A) polymerase.
Enzyme Commission Number
EC 2.7.7.8
CAS No.
9014-12-4
Unit Definition
One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37°C. Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol
Storage
−70°C
Synonyms
PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4

"Polynucleotide phosphorylase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1908 Native Bacillus stearothermophilus Polynucleotide Phosphorylase EC 2.7.7.8 9014-12-4 Bacillus stearothermophilus Inquiry
NATE-0608 Polynucleotide Phosphorylase from Escherichia coli, Recombinant EC 2.7.7.8 9014-12-4 E. coli Inquiry
NATE-0609 Polynucleotide phosphorylase from Human, Recombinant EC 2.7.7.8 9014-12-4 E. coli Inquiry
EXWM-3292 polyribonucleotide nucleotidyltransferase EC 2.7.7.8 9014-12-4 Inquiry

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