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Protocatechuate 3,4-Dioxygenase from Bacteria, Recombinant

Cat No.
NATE-1028
Description
In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction:3,4-dihydroxybenzoate + O2↔ 3-carboxy-cis,cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.
Abbr
PCD, Recombinant (Bacteria)
Alias
PCD
Source
E. coli
Species
Bacteria
Applications
Useful for removal of protocatechuate derived from choline esterase determination.
Form
Solution
Enzyme Commission Number
EC 1.13.11.3
Activity
> 3 Units / mg
CAS No.
9029-47-4
Contaminants
NADPH oxidase < 0.01 % Alkaline phosphatase < 0.002 %
Molecular Weight
28 kD α subuit, 24 kD β subunit(SDS-PAGE)
pH Stability
5 to 10
Optimum pH
9
Optimum temperature
65°C
Thermal stability
< 60°C
Storage
1 - 10°C (do not freeze)
Synonyms
protocatechuate 3,4-dioxygenase; protocatechuate oxygenase; protocatechuic acid oxidase; protocatechuic 3,4-dioxygenase; protocatechuic 3,4-oxygenase; 9029-47-4; EC 1.13.11.3; PCD
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Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0509
EC No.EC 1.13.11.3
CAS No.9029-47-4
SourcePseudomonas sp.
Related Services
Related Protocols
protocatechuate_3,4-dioxygenase -Enzymatic Assay Protocol
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