Enzymes for Research, Diagnostic and Industrial Use

Reverse Transcriptase from Moloney Murine Leukemia Virus, Recombinant

Cat No.
This Reverse Transcriptase has exceptionally strong strand displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile and well-suited for applications requiring full-length cDNA such as preparation of cDNA libraries and other techniques involving first strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR). It can be used for performing a reverse transcription reaction with any RNA template including GC-rich templates and RNAs with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H Minus. Because of the excellent extension capability of PrimeScript Reverse Transcriptase, preparation of cDNA can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
RT, Recombinant (Moloney Murine Leukemia Virus)
E. coli
Moloney Murine Leukemia Virus
RT-PCR; First strand cDNA synthesis; cDNA probe preparation; Synthesis of cDNA libraries with a high proportion of full-length cDNAs.
Product Overview
1. Strong strand displacement and extension capability: Capable of synthesizing long and full-length cDNA molecules (up to 12 kb); 2. Preparation of cDNA at 42°C results in lower background and higher cDNA yields because the lower temperature does not promote RNA degradation; 3. Outstanding accuracy: Lowest error rate among five commercially available reverse transcriptases tested; 4. Highly specific: Low rates of non-specific annealing, even on incompletely denatured RNA; 5. Works on challenging templates: Excellent results even with GC-rich templates and templates with high levels of secondary structure 6. Highly sensitive: use less of your precious RNA samples; 7. Demonstrated success with reverse transcription reaction times of 30 min. (60 min. for longer transcripts).
Unit Definition
One unit is the amount of the enzyme that incorporates 1 nmol of [3H]dTTP in 10 minutes at 37°C, with poly (rA), oligo (dT) 12-18 as the primer-template.
Storage at-20°C
5X Buffer (for cDNA synthesis) 250 mM Tris-HCl, pH 8.3 375 mM KCl 15 mM MgCl2 Reaction mixture for unit definition: 50 mM: Tris-HCl, pH 8.3 75 mM: KCl 8mM: MgCl2 10 mM: DTT 20 ug/mL: (ra)n (dT)12-18 0.5 mM: [3H]dTTP 0.1%: NP-40 Storage Buffer Composition 200 mM Tris-HCl, pH 7.8 100 mM NaCl 1 mM EDTA 1 mM DTT 50% Glycerol (v/v)
Reverse transcriptase; RT
Download Datasheet:

“RT” Total Products Page

Related Products
EC No.
CAS No.9068-38-6
SourceE. coli
Online Inquiry
*E-mail Address:
Country or Region:
*Products or Services Interested:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy  Terms and Conditions
Copyright ©2011 - 2018 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country:
Click unfold / close
Inquiry Basket
Delete selected Check Out
Decide to move out of the shopping cart??

Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product