Description
Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5, 10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen. sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
Abbr
SAO, Recombinant (E. coli)
Appearance
Yellow lyophilizate
Enzyme Commission Number
EC 1.5.3.1
Activity
> 15 U/mg lyophilizate
Contaminants
catalase < 0.5% glucose oxidase < 1.0 x 10^-5%
Molecular Weight
ca. 49 kDa
Michaelis Constant
4.7 x 10^-3 M (sarcosine)
Structure
monomer of 43 kDa (SDS-PAGE) one mole of FAD per mole of enzyme
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.7.
Thermal stability
below 55°C
Stability
Stability (liquid form) stable at 37°C for at least two weeks Stability (powder form) stable at 30°C for at least one month
Inhibitors
Zn2+, Cu2+, Hg2+, Ag+
Synonyms
Sarcosine Oxidase; EC 1.5.3.1; SAO
