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Sequencing Grade Chymotrypsin from Human, Recombinant

Cat No.
NATE-1894
Description
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Source
E. coli
Species
Human
Applications
1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
Form
Lyophilized
Enzyme Commission Number
EC 3.4.21.1
Activity
>1500 unit/mg protein
CAS No.
9004-07-3
Molecular Weight
26,950 Da
Purity
> 98% by HPLC
Storage
Recombinant Chymotrypsin lyophilized should be stored under 2°C-8°C in sealed container. It is stable within 24 months. After dissolved, it should be stored under -20°C. It is stable within 24 months and above 90% activity remained after 3 times repeated freezing and thawing.
Preparation Instructions
For disolving buffer, it is 50mM HAC,to get the terminal concentration of 0.5μg/μl, used once time or stored below -20°C. For the digestion buffer, in general, it is 100mM NH4HCO3 containing 5mM CaCl2, at 30°C, to dissolve the aim protein and then added chymotrypsin (in general, W:W=1:20-1:100). Self digestion may occur if temperatures above 37°C.
Synonyms
EC 3.4.21.1; chymotrypsins A and B; alpha-chymar ophth; avazyme; chymar; chymotest; enzeon; quimar; quimotrase; alpha-chymar; alpha-chymotrypsin A; alpha-chymotrypsin; Chymotrypsin; α-Chymotrypsin; Sequencing Grade Recombinant Chymotrypsin; Sequencing Grade Chymotrypsin
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1890
EC No.EC 3.4.21.1
CAS No.9004-07-3
SourceE. coli
CatalogDIGS-227
ProductNameChymotrypsin
EC No.EC 3.4.21.1
CAS No.9004-07-3
Source
CatalogBIO-1012
ProductNameChymotrypsin
EC No.
CAS No.9004-07-3
Source
CatalogEXWM-4093
ProductNamechymotrypsin
EC No.EC 3.4.21.1
CAS No.9004-07-3
Source
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Realted Protocols
a-CHYMOTRYPSIN -Enzymatic Assay Protocol
a-chymotrypsin -Enzymatic Assay Protocol
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