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Succinyl-CoA synthetase from Prokaryote, Recombinant

Cat No.
NATE-0916
Description
In enzymology, a succinate-CoA ligase (ADP-forming) (EC 6.2.1.5) is an enzyme that catalyzes the chemical reaction: ATP + succinate + CoA ↔ ADP + phosphate + succinyl-CoA. The 3 substrates of this enzyme are ATP, succinate, and CoA, whereas its 3 products are ADP, phosphate, and succinyl-CoA. This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. This enzyme participates in 4 metabolic pathways: Citric acid cycle, propanoate metabolism, c5-branched dibasic acid metabolism, and reductive carboxylate cycle (CO2 fixation).
Abbr
A-SCS, Recombinant (Prokaryote)
Alias
A-SCS; STK
Source
Prokaryote
Applications
High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 6.2.1.5
Activity
8.7 U/mg protein at pH 8.4 and 25°C.
CAS No.
9080-33-5
Molecular Weight
~ 30,843 and ~ 41,393 Da
Isoelectric point
~ 5.9
Unit Definition
One Unit of succinyl-CoA synthetase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
8.4
Optimum temperature
25°C
Stability
> 2 years at 4°C.
Storage
Store at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM glycylglycine buffer, pH 8.4 containing 10 mM MgCl2. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
CoA ligase (ADP-forming); succinyl-CoA synthetase (ADP-forming); succinic thiokinase; succinate thiokinase; succinyl-CoA synthetase; succinyl coenzyme A synthetase (adenosine diphosphate-forming); succinyl coenzyme A synthetase; A-STK (adenin nucleotide-linked succinate thiokinase); STK; A-SCS; succinate-CoA ligase (ADP-forming); EC 6.2.1.5
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CatalogEXWM-5707
EC No.EC 6.2.1.5
CAS No.9080-33-5
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