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tRNA (cytidine32/uridine32-2'-O)-methyltransferase

Cat No.
EXWM-1801
Description
In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine32 in tRNA. No methylation of cytosine34 in tRNALeu(CAA). In vitro the enzyme 2-O-methylates cytidine32 of tRNASer1 and uridine32 of tRNAGln2.
Form
Liquid or lyophilized powder
Enzyme Commission Number
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Synonyms
YfhQ; tRNA:Cm32/Um32 methyltransferase; TrMet(Xm32); TrmJ
Reaction
(1) S-adenosyl-L-methionine + cytidine32 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA; (2) S-adenosyl-L-methionine + uridine32 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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