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Transglutaminase 7 from Human, Recombinant

Cat No.
NATE-1737
Description
Transglutaminase 7 is based on the TGM7-gene on plasmid pCRII-hTGz cl.14 (isolated by Daniel Aeschlimann), corrected by the insertion of a C at position 1169. It is N-terminally fused to a hexahistidine-tag.
Abbr
TG 7, Recombinant (Human)
Alias
TG7
Source
E. coli
Species
Human
Applications
The transglutaminase 7 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. The transglutaminase 7 may also be used for immunoprecipitation.
Appearance
White lyophilized solid.
Package
250 μg
Form
The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 1000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
CAS No.
80146-85-6
Molecular Weight
81 kDa
Purity
> 90 % (visually by SDS-PAGE)
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Activators
Add 10 mM Ca2+ to activate transglutaminase.
Storage
Store at ≤ -20°C.
Store working aliquots at ≤ -20°C. Avoid repeated freezing and thawing.
Delivery at ambient temperature is possible
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.
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Related Protocols
transglutaminase -Enzymatic Assay Protocol
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