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Trehalase from Prokaryote, Recombinant

Cat No.
NATE-1126
Description
Trehalase is a glycoside hydrolase enzyme located in on the brush border of the small intestine that catalyzes the conversion of trehalose to glucose. It is found in most animals. The non-reducing disaccharide trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is one of the most important storage carbohydRates, which is present in almost all forms of life except mammals. The disaccharide is hydrolyzed into two molecules of glucose by the enzyme trehalase. There are two types of trehalases found in Saccharomyces cerevisiae, viz. neutral trehalase (NT) and acid trehalase (AT) classified according to their pH optima. NT has an optimum pH of 7.0, while that of AT is 4.5. Recently it has been reported that more than 90% of total AT activity in S. cerevisiae is extracellular and cleaves extracellular trehalose into glucose in the periplasmic space.
Abbr
Trehalase, Recombinant (Prokaryote)
Source
Prokaryote
Applications
High purity Trehalase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
Ammonium sulphate suspension.
Enzyme Commission Number
EC 3.2.1.28
Activity
303 U/mg protein at pH 5.5 and 40°C; 61 U/mg protein at pH 7.0 and 40°C.
CAS No.
9025-52-9
Molecular Weight
~ 63,636
Isoelectric point
~ 5.6
Unit Definition
One Unit of trehalase is defined as the amount of enzyme required to produce two μmoles of glucose from one μmole of trehalose per mwwinute.
Optimum pH
5.5
Thermal stability
up to 40°C
Stability
> 4 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM sodium maleate buffer, pH 5.5 containing 0.5 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
α,α-Trehalose glucohydrolase; Trehalase; EC 3.2.1.28; 9025-52-9; α,α-trehalase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0717
EC No.EC 3.2.1.28
CAS No.9025-52-9
SourcePorcine kidney
CatalogEXWM-3892
ProductNameα,α-trehalase
EC No.EC 3.2.1.28
CAS No.9025-52-9
Source
Realted Service
Realted Protocols
trehalase -Enzymatic Assay Protocol
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