Description
In enzymology, an acetylxylan esterase (EC 3.1.1.72) is an enzyme that catalyzes a chemical reaction, the deacetylation of xylans and xylo-oligosaccharides. This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds.
Abbr
Xylanase & Acetyl xylan esterase, Recombinant (Clostridium thermocellum)
Species
Clostridium thermocellum
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.8 and 3.1.1.72
Molecular Weight
72.5 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Xylans, such as oat spelt xylan and arabinoxylan (GH11), and removes acetate from acetylated xylan (CE4)
Optimum temperature
65 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Acetylxylan esterase; EC 3.1.1.72; 188959-24-2; 9000-82-2