Description
In enzymology, a xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1.151) is an enzyme that catalyzes the chemical reaction: xyloglucan + H2O rightleftharpoons xyloglucan oligosaccharides. Thus, the two substrates of this enzyme are xyloglucan and H2O, whereas its product is xyloglucan oligosaccharides. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds.
Abbr
XEG, Recombinant (Paenibacillus sp.)
Applications
High purity recombinant Xyloglucanase (Paenibacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.151
Activity
45 U/mg protein (on tamarind xyloglucan) at pH 6.0 and 70°C.
Molecular Weight
~ 108 kDa
pH Stability
5.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Unit Definition
One Unit of xyloglucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from xyloglucan (5 mg/mL) in sodium acetate buffer (100 mM) pH 6.0.
Stability
> 2 years at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 6.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
xyloglucan-specific endo-beta-1,4-glucanase; EC 3.2.1.151; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase; XEG; XH; xyloglucan endo-beta-1,4-glucanase; xyloglucanase; xyloglucanendohydrolase; 1,4-beta-D-glucan glucanohydrolase
